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Thiostrepton-resistant mutants of Bacillus subtilis: Localization of resistance to the 50S subunit

✍ Scribed by Pestka, Sidney ;Weiss, David ;Vince, Robert ;Wienen, Bertold ;Stöffler, Georg ;Smith, Issar

Publisher
Springer
Year
1976
Tongue
English
Weight
692 KB
Volume
144
Category
Article
ISSN
0026-8925

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✦ Synopsis

A number of thiostrepton-resistant mutants of Bacillus subtilis were obtained. The thi mutations map proximally to strA. Effects of thiostrepton on polyphenylalanine synthesis with ribosomes of S-100 fractions from parent and mutant strains indicated that resistance was localized to the ribosomes. Furthermore, effects of thiostrepton on binding of [3H]GTP to ribosomes and 50S subunits from thiostrepton-sensitive and -resistant strains localized the site of resistance to the 50S subunit. In addition, revertants from thiostrepton-resistance to thiostrepton-sensitivity were obtained. Ribosomes and 50S subunits from these thiostrepton-sensitive revertants were sensitive to thiostrepton similar to parental sensitive B. subtilis.

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