Thin layer gel chromatography Part 2. Molecular weight determination of proteins
β Scribed by D. Jaworek
- Book ID
- 112723588
- Publisher
- Springer
- Year
- 1970
- Tongue
- English
- Weight
- 598 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0009-5893
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The permeation properties of chromatographic gels with large pore sizes have been examined by the use of selected, water-soluble proteins. We observed that plots of Stokes radius vs erf-\* (1 -Ka) of Sepharose gels were nonlinear, probably reflecting the presence of two classes of pore sizes of the
Thin-layer gel chromatography of proteins in the presence of the mild detergents, sodium deoxycholate and Triton X-fO0, or in the presence of a denaturing detergent, sodinm dodecyl sulfate, is described in terms of application to (glyco)proteins of membrane origin. En these detergent systems, a near