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Thin-layer gel chromatography of proteins in mild and denaturing detergents

✍ Scribed by James F. Collawn Jr.; David J. Cox; Larry M. Hamlin; Wayne W. Fish

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 683 KB
Volume: 157
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)92337-9

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✦ Synopsis

Thin-layer gel chromatography of proteins in the presence of the mild detergents, sodium deoxycholate and Triton X-fO0, or in the presence of a denaturing detergent, sodinm dodecyl sulfate, is described in terms of application to (glyco)proteins of membrane origin. En these detergent systems, a nearly linear relationship was observed between the value of the distribution coefficient of a protein and the protein's equivalent hydrodynamic radius. The fractionation properties of the gel employed in this investigation appeared to be different in T&on X-100 than in the other two detergent systems. The &ration and replica-staining of glycoproteins separated in the presenti of detergent is also discussed.

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