Thin-layer composite enzyme electrodes for glucose determinations

✍ Scribed by Thomas A. Zawodzinski Jr.; Carmen Campbell; John Davey; Shimshon Gottesfeld; James Serach; Mahlon Wilson; Judith Rishpon

• Book ID: 102832995
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 763 KB
• Volume: 7
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.1140071108

✦ Synopsis

We have prepared amperometric glucose sensitive electrodes with a composite active layer consisting of Nafion, glucose oxidase, and carbon-supported platinum particles. A particularly advantageous configuration results from the use of such a film on a gas diffusion electrode. We demonstrate that the gas diffusion electrode configuration enables us to supply oxygen from the back of the electrode, thus providing the capability of operating the sensor independent of dissolved oxygen. The insensitivity to solution oxygen concentration has been demonstrated by monitoring the glucose response of the electrode after extensive deoxygenation of solution. Cast composite layers yield mechanically robust coatings with high enzyme loadings, and thus high sensitivity to glucose. The electrode responds rapidly and is stable over a long period (90% activity after more than half a year) when stored in solution. We have optimized the composition of the sensitive layer with respect to Nafion to CjPt ratio and enzyme loading.