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Thin-layer chromatography of urinary 17-oxosteroids using dansylhydrazine as a prelabelling reagent

✍ Scribed by Zdena Tomsová

Book ID
107996876
Publisher
Elsevier Science
Year
1991
Weight
108 KB
Volume
570
Category
Article
ISSN
0378-4347

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✍ Kazufusa Shinomiya; Shigeyuki Yamanashi; Toshio Imanari 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 302 KB

Chondroitin sulfate isomers (ChS isomers) were digested with chondroitinase ABC, and the unsaturated disaccharides produced were converted into fluorescent dansylhydrazine derivatives and analyzed by HPLC. The proposed method was applied to the determination of relative amounts of ChS isomers in nor