A micro thin layer chromatography technique for urinary and serum amino acids using fluorescamine as a staining reagent

4-(5,6-Dimethoxy-2-phthalimidinyl)-2-met

4-(5,6-Dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl Chloride as a Fluorescent Labeling Reagent for Determination of Amino Acids in High-Performance Liquid Chromatography and Its Application for Determination of Urinary Free Hydroxyproline

✍ Yasuto Tsuruta; Hirofumi Inoue 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 106 KB

A highly sensitive fluorescent labeling reagent, 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride (DMS-Cl), for determination of amino acids by HPLC has been developed. DMS-Cl reacted with amino acids in the basic medium to produce the corresponding fluorescent sulfonamides (excit