Thin-layer chromatography of purine bases and deoxyribonucleoside analogues. IV

✍ Scribed by R. Cardinaud

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 277 KB
• Volume: 154
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)98493-0

✦ Synopsis

In a study of the specificity of Laciobacillvs helveticus nucleoside deoxyribosyltrqnsferasel, it was observed that 5-aminoimidazoIe carboxamide was a sub&a&, showing that apart from the imidazole ring a complete fused six-membered ring was not necessary. On the other hand, imidazole did not react, sugestig a role for some substituents at the 4 and/or 5-position in imidazole. In krther studies on the specificity of nucleoside deoxyribosyltransferase I and II, we measured the RF valuqs of a new series of purine analogues and imidazole derivatives in the six developing solvents already used in our previous studies3. The RF values of nine newly synthesized deoxyribonuckosides were also measured. ExPERrMENTAL Materials Most of the analogues were commercial compounds purchased from 'Aldrich (Milwaukee, Wk.