Thin-layer chromatography of neutral and acidic sugars from plant cell wall polysacharides
β Scribed by J.P Metraux
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 204 KB
- Volume
- 237
- Category
- Article
- ISSN
- 1873-3778
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β¦ Synopsis
Thin-layer chromatography (TLC) has been used to separate carbohy-dratesx4. However, these methods have been of limited use for the separation of mixtures of a large number of sugars, and so far no appropriate TLC technique exists which allows for a complete separation of all monomers obtained after hydrolysis of plant cell walls. The present report describes a two-dimensional thin-layer technique on unimpregnatecl cellulose for the separation of seven neutral and two acidic sugars such as those found in hydrolysates of plant cell wall polysaccharides.
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A method for quantitative determination ofacidic phospholipids by thin-layer chromatography (TLC) followed by densitometry is described. The total lipids were separated into neutral and acidic fractions by DEAE-Sephadex column chromatography. A clear-cm separation of acidic phospholipids was achieve