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Thin-layer chromatography of amino acid hydantoins

✍ Scribed by Tateo Suzuki; Kieko Komatsu; Katura Tuzimura

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
583 KB
Volume
80
Category
Article
ISSN
1873-3778

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✦ Synopsis

Amino acid hydaneoins were detected directly in amounts of 0.2-O.Spg with tevt.-bueyl hypochlorite.

The amino acid hydantoins' were separated by thin-layer chromatography using the following solve@ systems: (I) chloroform-ethanol-formic acid (2: 1: 1); (II) rz-but.yl acetate-ethanol-formic acid (12:2: 1); (III) dichloroethaneethanol-formic acid (80: 20: 1); and (IV) n-butanol-acetic acid-water (15: 2: 5). The terminal amino groups of several proteins were detected successfully.

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