Thin-layer chromatographic analysis of diphenylmethane and its regioisomeric1 diphenylmethyl alcohols

✍ Scribed by Otis J. Bouwsma; Andrew W. Stocklinski

• Book ID: 104145337
• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 343 KB
• Volume: 154
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)98492-9

✦ Synopsis

The metabolic profile of diphenylmethane is being re-investigated in these laboratories* as an extension of the stimulating work by Stroud3, Butler et d4*', Leib-man6, Robinson and Williams7*8, Eliisong, Hespe et aLEo and Focht and AlexandeP*" (schemes l-3). Based on their reports and previous studies on the metabolism of fluorene13-16, a thin-layer chromatographic (TLC) assay was desired which would simultaneously separate diphenylmethane, benzhydrol, o-hydroxydiphenylmethane, and p-hydroxydiphenylmethane.

While several thin-layer methods are capable of chromatographing benzhydrol in the presence of other compounds6~'1~12~17-*o, these systems do not adequately separate it from either orrho-or para-hydroxydiphenylmethane. This paper describes a rapid thin-layer method which can be used to separate and qualitatively detect benzhydrol in the presence of diphenyhnethane, uhydrdxydiphenylmethane, and p-hydroxydiphenylmethane.