Release of virus-like particles by osmot
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Pablo Γlvarez; Miguel SΓ‘nchez; MarΓa Molina; CΓ©sar Nombela
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Article
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1995
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Springer-Verlag
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English
β 376 KB
A yeast system based on a S. cerevisiae mutant strain deficient in cell integrity, was used to achieve the rclcasc of yeast expressed virus-like particles (vI.Ps) by simple osmotic shock. Yeast cells, grown in the prcscnce of IM sorbitol, lysed and rcleac;ed the intracellular content upon being tran