✦ LIBER ✦
Release of virus-like particles by osmotic shock from a mutant strain of yeast deficient in cell integrity
✍ Scribed by Pablo Álvarez; Miguel Sánchez; María Molina; César Nombela
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 376 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0951-208X
No coin nor oath required. For personal study only.
✦ Synopsis
A yeast system based on a S. cerevisiae mutant strain deficient in cell integrity, was used to achieve the rclcasc of yeast expressed virus-like particles (vI.Ps) by simple osmotic shock. Yeast cells, grown in the prcscnce of IM sorbitol, lysed and rcleac;ed the intracellular content upon being transfcmzd to non-osmotically stahilizd medium. The release of VLPs was followcrl by Western blotting determination of the pl protein.