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Thesmt-0mutation which abolishes mating-type switching in fission yeast is a deletion

✍ Scribed by Unnur Styrkársdóttir; Richard Egel; Olaf Nielsen

Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 303 KB
Volume: 23
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00352020

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✦ Synopsis

Mating-type switching in the fission yeast, S. pombe, is initiated by a DNA double-strand break (DSB) between the mat1 cassette and the H1 homology box. The mat1-cis-acting mutant, smt-0, abolishes mating-type switching and is shown here to be a 263-bp deletion. This deletion starts in the middle of the H1 homology box, 31 bp from the site of the DSB, and extends into the flanking region distal to mat1. The sequence of the region distal to H1 in the wild-type is also presented. In this region we observe a bias in the distribution of purine residues between the two DNA strands.

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