Mating-type switching in the fission yeast, S. pombe, is initiated by a DNA double-strand break (DSB) between the mat1 cassette and the H1 homology box. The mat1-cis-acting mutant, smt-0, abolishes mating-type switching and is shown here to be a 263-bp deletion. This deletion starts in the middle of
Mating-type switching in yeast is induced by thymine nucleotide depletion
β Scribed by Kunz, B. A. ;Taylor, G. R. ;Haynes, R. H.
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 339 KB
- Volume
- 199
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Thymidylate biosynthesis was inhibited in a haploid heterothallic strain of Saccharomyces cerevisiae. When the treated cells were mixed with a haploid strain of the same mating-type, there was an increase in the recovery of diploid colonies. Genetic and biochemical analyses demonstrated that the diploid clones arose as a consequence of induced mating-type interconversion.
π SIMILAR VOLUMES
The mating type of fission yeast is determined by the mat1 locus on chromosome II. The sequence content of this locus, and hence the mating type, is switched in a strictly regular pattern by transposition from one of two unexpressed mating type sequences. The expressed and the two silent sequences a
Mating-type switching in homothallic clones of the fission yeast, Schizosaccharomyces pombe, appears to follow the same route as previously found for "mutations" from homothallism to heterothallic strains. A copy of mat2-P is transposed to and inserted at rnatl, where it functionally replaces the rn