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Thermotolerance in Saccharomyces cerevisiae: the Yin and Yang of trehalose

✍ Scribed by Mike A Singer; Susan Lindquist


Book ID
114153105
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
642 KB
Volume
16
Category
Article
ISSN
0167-7799

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Saccharomyces cerevisiae cells are able to grow using trehalose as a sole source of carbon and energy. However, the biomass yield obtained with trehalose was higher, and the specific growth rate lower, than that obtained with glucose or maltose. The respiratory inhibitor antimycin A prevented cell g

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A simple, sensitive and nonlaborious enzyme-based method has been developed for determination of both trehalose and glycogen in yeast cells. The method is based on extraction of trehalose and glycogen into a \(40 \mathrm{~mm}\) acetate buffer ( \(\mathbf{p H} 4.8\) ) by mechanical disintegration of