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Kinetics and Energetics of Trehalose Transport in Saccharomyces cerevisiae

✍ Scribed by Boris U. Stambuk; Pedro S. De Araujo; Anita D. Panek; Ramon Serrano


Book ID
115132278
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
661 KB
Volume
237
Category
Article
ISSN
1432-1327

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Determination of Intracellular Trehalose
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A simple, sensitive and nonlaborious enzyme-based method has been developed for determination of both trehalose and glycogen in yeast cells. The method is based on extraction of trehalose and glycogen into a \(40 \mathrm{~mm}\) acetate buffer ( \(\mathbf{p H} 4.8\) ) by mechanical disintegration of