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Thermostabilization of lactate oxidase by random mutagenesis

โœ Scribed by Hirotaka Minagawa; Noriyuki Nakayama; Shinya Nakamoto

Publisher
Springer Netherlands
Year
1995
Tongue
English
Weight
391 KB
Volume
17
Category
Article
ISSN
0141-5492

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โœฆ Synopsis

A lactate oxidase (LOD) gene from Aerococcus viriduns was cloned and sequenced to generate thermostable LOD. One mutant LOD selected from a set of variants created by random mutagenesis had a half life of 6.2 min at 65ยฐC approximately three times longer than that of the wild type LOD. This mutant exhibited an Asn to Asp point mutation at position 212 in the amino acid sequence.

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