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Thermostability and electron transfer activity of the ferredoxin from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii

✍ Scribed by Shigetoshi Aono; Norio Fukuda; Ichiro Okura

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
623 KB
Volume
95
Category
Article
ISSN
1381-1169

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✦ Synopsis

The Azorobucrer-type 7Fe ferredoxin from Bacillus schlegelii was active as an electron carrier in the reduction system of cyt. c consisting of NADPH, FNR and cytochrome c (cyt. c). The only [ 3Fe_4S] cluster in the Fd molecule acts as the active site for the reduction of cyt. c. The activity of the ferredoxin once increased by IO,20 and 20% by heat treatment at 60,80 and 90°C respectively. The increasing of the activity is caused by the interconversion of the [4Fe-4S] cluster to the [ 3Fe-4S] cluster with increasing of the [ 3Fe_4S] cluster in the concentration.

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