The Thermophilic CotA Laccase from Bacillus subtilis: Bioelectrocatalytic Evaluation of O2 Reduction in the Direct and Mediated Electron Transfer Regime
✍ Scribed by Thomas Beneyton ; Yvonne Beyl ; Dmitrii A. Guschin; Andrew D. Griffiths; Valerie Taly; Wolfgang Schuhmann
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 544 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1040-0397
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The thermophilic bacterial laccase CotA from Bacillus subtilis adsorbed on graphite electrodes enables monitoring of the temperature dependent direct biocatalytic O~2~ reduction. Its entrapment in two different Os‐complex modified redox hydrogels is the basis for mediated bioelectroreduction of O~2~. Besides the temperature and pH dependence of the bioelectrocatalytic response chloride and fluoride inhibition studies demonstrate that the Os‐complexes are bound to the T1 copper centre of the enzyme. The interaction between CotA and the Os‐complex modified polymers prevents inhibition by chloride ions.