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Thermoplasma acidophilum: Intracellular pH and potassium concentration

✍ Scribed by Searcy, Dennis G.


Book ID
122538291
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
539 KB
Volume
451
Category
Article
ISSN
0304-4165

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A flow cytometric assay has been developed for determination of intracellular free potassium concentration ([K 1 ] i ). Investigated cells, loaded with the fluorescent pH indicator 28,7bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), are incubated in the presence of nigericin, and the intra