𝔖 Bobbio Scriptorium
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A novel method for measuring intracellular pH and potassium concentration

✍ Scribed by C. L. Bashford; G. Alder; K. J. Micklem; C. A. Pasternak

Book ID
112491696
Publisher
Springer
Year
1983
Tongue
English
Weight
690 KB
Volume
3
Category
Article
ISSN
0144-8463

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A new flow cytometric method was developed for measuring the intracellular pH (pHi) of mammalian cells using a fluorescent pH indicator dye 2',7-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF). Emission intensities (or their ratios) measured from BCECF-loaded cells can be converted into abs