A new flow cytometric method was developed for measuring the intracellular pH (pHi) of mammalian cells using a fluorescent pH indicator dye 2',7-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF). Emission intensities (or their ratios) measured from BCECF-loaded cells can be converted into abs
A novel method for absolute calibration of intracellular pH indicators
β Scribed by D. A. Eisner; N. A. Kenning; S. C. O'Neill; G. Pocock; C. D. Richards; M. Valdeolmillos
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 635 KB
- Volume
- 413
- Category
- Article
- ISSN
- 0031-6768
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