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Thermodynamics of solid-to-solid conversion and application to enzymic peptide synthesis

✍ Scribed by P.J. Halling; U. Eichhorn; P. Kuhl; H.-D. Jakubke


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
634 KB
Volume
17
Category
Article
ISSN
0141-0229

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We have studied a thermolysin-catalyzed solidto-solid dipeptide synthesis using equimolar amounts of Z-Gln-OH and H-Leu-NH 2 as model substrates. The high substrate concentrations make this an effective alternative to enzymatic peptide synthesis in organic solvents. Water content was varied in the r

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A systematic study of thermolysin-catalyzed solid-to-solid peptide synthesis using Z-Gln and Leu-NH 2 as model substrates was carried out. The aim was to extend the kinetic knowledge of this new reaction system involving highly concentrated substrate mixtures with little water (10% to 20% w/w). Preh