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Thermodynamics of Ligand Binding to Acyl-Coenzyme A Binding Protein Studied by Titration Calorimetry †

✍ Scribed by Færgeman, Nils Joakim; Sigurskjold, Bent W.; Kragelund, Birthe B.; Andersen, Kim V.; Knudsen, Jens

Book ID
126161359
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
446 KB
Volume
35
Category
Article
ISSN
0006-2960

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A competition-based method is used for the determination of the thermodynamic parameters for a low-affinity ligand binding reaction by isothermal titration calorimetry. This method is based on the coupling of a high-affinity ligand to the binding of the low-affinity ligand. Results are presented for