Investigation of Ligand Binding to the Multidrug Resistance Protein EmrE by Isothermal Titration Calorimetry
β Scribed by Sikora, Curtis W.; Turner, Raymond J.
- Book ID
- 119918960
- Publisher
- Biophysical Society
- Year
- 2005
- Tongue
- English
- Weight
- 196 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0006-3495
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π SIMILAR VOLUMES
Bacterial Protein A (PrtA) and Protein G (PrtG) are widely used for affinity purification of antibodies. An understanding of how PrtA and PrtG bind to different isotypes of immunoglobulin type G (IgG) and to their corresponding Fc fragments is essential for the development of PrtA and PrtG mimetic l
A competition-based method is used for the determination of the thermodynamic parameters for a low-affinity ligand binding reaction by isothermal titration calorimetry. This method is based on the coupling of a high-affinity ligand to the binding of the low-affinity ligand. Results are presented for