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Thermodynamics of DNA Duplex Formation: A Biophysical Chemistry Laboratory Experiment

✍ Scribed by Howard, Kathleen P.

Book ID
120492805
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
89 KB
Volume
77
Category
Article
ISSN
0021-9584

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The rates of duplex formation for two octamers of DNA (5β€² d-CACGGCTC/5β€² d-GAGCCGTG and 5β€² d-CACAGCAC/5β€² d-GTGCTGTG), the homologous RNA, and both sets of hybrids in 1 M NaCl buffer have been measured using stopped-flow spectroscopy. In addition, the thermodynamic parameters, Ξ”HΒ°and Ξ”SΒ°, have been de