We show that denatured DNA from Tetrahymena mitochondria or phage TT, partially renatures during centrifugation in NaI equilibrium density gradients. This makes these gradients unsuitable for the analysis of single-stranded complementary nucleic acids, especially if their complexity and mole percent
Thermal denaturation and renaturation of DNA molecules
โ Scribed by M.L.C. Leung; B.Y. Tong; F.Y. Wu
- Book ID
- 103770694
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 160 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0375-9601
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The kinetics of renaturation of heat-or formamidedenatured DNA have been studied by following the change of optical density at a constant temperature. Solvents of different ionic strength and various DNA samples have been used. At the lower ionic strength studied, the reaction follows second-order k
The structure of renatured T4 DNA has been studied by CsCl density-gradient centrifugation. It has, been found that the products of the reaction differ, depending on the method used for denaturation of the DNA. If denaturation is carried out without taking precautions to prevent chain degradation, f