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Renaturation of denatured DNA in NaI gradients

โœ Scribed by R. A. Clegg; P. Borst

Book ID
104738918
Publisher
Springer
Year
1974
Tongue
English
Weight
266 KB
Volume
1
Category
Article
ISSN
0301-4851

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โœฆ Synopsis

We show that denatured DNA from Tetrahymena mitochondria or phage TT, partially renatures during centrifugation in NaI equilibrium density gradients. This makes these gradients unsuitable for the analysis of single-stranded complementary nucleic acids, especially if their complexity and mole percent G + C are low.

๐Ÿ“œ SIMILAR VOLUMES

Kinetics of renaturation of denatured DN
Kinetics of renaturation of denatured DNA. I. Spectrophotometric results
โœ Juan A. Subirana; Paul Doty ๐Ÿ“‚ Article ๐Ÿ“… 1966 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 963 KB

The kinetics of renaturation of heat-or formamidedenatured DNA have been studied by following the change of optical density at a constant temperature. Solvents of different ionic strength and various DNA samples have been used. At the lower ionic strength studied, the reaction follows second-order k

Kinetics of renaturation of denatured DN
Kinetics of renaturation of denatured DNA. II. Products of the reaction
โœ Juan A. Subirana ๐Ÿ“‚ Article ๐Ÿ“… 1966 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 620 KB

The structure of renatured T4 DNA has been studied by CsCl density-gradient centrifugation. It has, been found that the products of the reaction differ, depending on the method used for denaturation of the DNA. If denaturation is carried out without taking precautions to prevent chain degradation, f