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Thermal and pH stabilities of alkaline phosphatase from bovine intestinal mucosa: a FTIR study

✍ Scribed by Laurence de La Fournière; Olivier Nosjean; René Buchet; Bernard Roux

Book ID
113269559
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
603 KB
Volume
1248
Category
Article
ISSN
0167-4838

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Alkaline phosphatase (EC 3.1.3.1) from bovine intestinal mucosa (BIAP) is an homodimeric metalloenzyme, containing one Mg 2؉ and two Zn 2؉ ions in each active site. ApoBIAP, prepared using ion-chelating agents, exhibited a dramatic decrease of its hydrolase activity, concomittant to conformational c