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An Infrared Study of the Thermal and pH Stabilities of the GPI-Alkaline Phosphatase from Bovine Intestine

✍ Scribed by Muriel Bortolato; Françoise Besson; Bernard Roux


Book ID
115589773
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
195 KB
Volume
292
Category
Article
ISSN
0006-291X

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✍ Muriel Bortolato; Françoise Besson; Bernard Roux 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 286 KB 👁 1 views

Alkaline phosphatase (EC 3.1.3.1) from bovine intestinal mucosa (BIAP) is an homodimeric metalloenzyme, containing one Mg 2؉ and two Zn 2؉ ions in each active site. ApoBIAP, prepared using ion-chelating agents, exhibited a dramatic decrease of its hydrolase activity, concomittant to conformational c