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Theory of a rapid method for determining molecular weights of giant molecules

✍ Scribed by Tadao Kotaka; Robert L. Baldwin

Publisher
Wiley (John Wiley & Sons)
Year
1969
Tongue
English
Weight
546 KB
Volume
7
Category
Article
ISSN
0006-3525

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✦ Synopsis

A new method is proposed for finding molecular weights from the boundary condition of the ultracentrifuge. The method is more rapid than the conventional Archibald method by a t least one order of magnitude and should be especially useful for giant molecules with small diffusion coefficients. It employs a preset concentration gradient.

A theoretical study of the method has been made by obtaining solutions to the Lamm equation analogous to the "mirror image" solution (for early times) and the Fourier expansion solution (for late times) of Mason and Weaver. Numerical examples of theoretical results are given, and the errors are discussed. Centrifugation in a preset linear gradient can also be used to reduce the time needed to reach equilibrium.

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