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A simple method for the isolation of undegraded giant RNA molecules

✍ Scribed by Steven T. Case; Bertil Daneholt

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 493 KB
Volume: 74
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90324-9

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✦ Synopsis

A simple technique is described whereby RNA molecules can be rapidly isolated in a high yield from preparative agarose gels. Slices from the preparative gel which contain the desired RNA species are made into an agarose plug. This provides for handling of the slices in such a way as to permit electrophoretic elution of RNA molecules into very small volumes. This technique is shown to be mild enough to permit, for the first time, the isolation of an undegraded preparation of 75 S RNA.

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