The substrate specificity of a camphor-induced cytochrome P-450 (P-450 cam ) was measured by using a new assay system: electrochemical control of P-450 cam activity by protein immobilization on an electrode. Immobilized P-450 cam showed the obvious substrate specificity for hydroxylation of the subs
Theoretical study of the substrate specificity of cytochrome P-450 isoforms
β Scribed by M. A. Grishina; A. A. Pogrebnoi; V. A. Potemkin; T. Yu. Zrakova
- Publisher
- Springer
- Year
- 2005
- Tongue
- English
- Weight
- 181 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0091-150X
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## Abstract Cytochrome P450 (P450) enzymes catalyze a variety of oxidation and some reduction reactions, collectively involving thousands of substrates. A general chemical mechanism can be used to rationalize most of the oxidations and involves a perfenyl intermediate (FeO^3+^) and oddβelectron che
We present an analysis of several molecular dynamics trajectories of substrate-bound cytochrome P450cam. Trajectories were calculated for the native substrate, camphor, as well as for the alternative substrates, norcamphor and thiocamphor. The system modeled consisted of the crystallographically res