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Theoretical Models of Spermine/DNA Interactions

✍ Scribed by BURT G. FEUERSTEIN; NAGARAJAN PATTABIRAMAN; LAURENCE J. MARTON


Book ID
118723405
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
133 KB
Volume
482
Category
Article
ISSN
0890-6564

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✍ Shalom Z. Hirschman; Marc Leng; Gary Felsenfeld πŸ“‚ Article πŸ“… 1967 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 360 KB

The effect of spermine upon the denaturation temperatuie (Tm) of DNA's of various base compositions has been found to depend upon both the base composition of the DNA and the pH of the solution. Measurement of the hydrogen ion titration curve of spermine as a function of temperature reveals that the

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The interaction of spermine with nucleic acids is simulated theoretically using refined semiempirical energy formulae and an advanced minimization procedure. Various nucleic acids are considered: model homopolymeric DNA's, a dodecamer (CGCGAATTCGCG) of type B-DNA, as well as a transfer RNA, tRNAPhe.

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## Abstract The energy of interaction of a spermine molecule with the A‐ and B‐forms of DNA has been calculated, assuming that the molecule of spermine is fixed in the narrow groove of the DNA helix with the formation of hydrogen bonds between the amino groups of spermine and the phosphate groups o