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Theoretical and practical considerations for the measurement of P-glycoprotein function in acute myeloid leukemia

✍ Scribed by Broxterman, H J; Lankelma, J; Pinedo, H M; Eekman, C A; Währer, D C R; Ossenkoppele, G J; Schuurhuis, G J


Book ID
110053535
Publisher
Nature Publishing Group
Year
1997
Tongue
English
Weight
261 KB
Volume
11
Category
Article
ISSN
0887-6924

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A bioassay was developed to assess P-glycoprotein (P-gp) function of peripheral blood natural killer (NK) cells and AML blasts during zosuquidar infusion. Cells were incubated with the fluorescent dye DiOC(2)(3) in the presence and absence of zosuquidar, and dye accumulation measured by flow cytomet