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Theoretical and experimental studies of electrostatic effects in reversed-phase liquid chromatography

✍ Scribed by Stephen G. Weber

Book ID
118995336
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
909 KB
Volume
36
Category
Article
ISSN
0039-9140

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This paper examines the reversed-phase liquid chromatographic behavior of ribonuclease A (RNase) using an n-butyl chemically bonded phase and a gradient of 10 mM H3PO4 and l-propanol. At a column temperature of 25 degrees C, a broad band followed by an overlapped late-eluting sharp peak is observed.