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The γ phosphorylase kinase gene,Phkg, maps to mouse Chromosome 5 nearGus

✍ Scribed by A. J. Maichele; J. S. Chamberlain

Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
513 KB
Volume
5
Category
Article
ISSN
0938-8990

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✦ Synopsis

Phosphorylase kinase is a multimeric regulatory enzyme in the glycogenolytic pathway. Interest in various types of phosphorylase kinase enzyme deficiency has focused attention on cloning and mapping the enzyme subunits. We report the mapping of the catalytic 7 subunit gene, Phkg, to mouse Chromosome (Chr) 5 near [3-glucuronidase (Gus), between alpha fetoprotein (Afp) and erythropoietin (Epo). In addition, PCR-based polymorphism assays have been developed for the human (EPO) and mouse erythropoietin genes, and a unique recombinant inbred strain distribution pattern has been defined for Epo, a distal anchor marker on mouse Chr 5.

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The BXD and AKXL panels of recombinant inbred mouse strains have been typed for the F liver protein alloantigen. The structural gene for F liver protein gene (Flp) is placed on the distal part of chromosome 5, between the known markers Bcd-1 and Gus-s. This excludes the possibility that F liver prot