𝔖 Bobbio Scriptorium
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The β-xylosidase of Thermomonospora

✍ Scribed by D. L. Ristroph; A. E. Humphrey

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
376 KB
Volume
27
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

This study is concerned with characterizing cell‐bound inducible β‐xylosidase produced by a strain of the thermophilic bacterial genus Thermomonospora. A crude preparation of this enzyme recovered from sonicated cells of this organism displayed high activity against paranitrophenyl‐β‐xylopyranoside over a pH range of 5.5‐7.7. The temperature optimum, based on a 30‐min assay of activity, at pH 6.5 was 70°C. The crude enzyme had a thermal half‐life of approximately 1 week at 55°C and pH 6.5. Xylose inhibited the enzyme. Values of K~m~ and V~max~ are estimated from the reaction rate data as 0.82 m__M__ and 8 U/L, respectively.

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