The Zebrafish: Genetics, Genomics and Informatics

✍ Scribed by H. William Detrich, Monte Westerfield and Leonard I. Zon (Eds.)

• Publisher: Academic Press
• Year: 2011
• Tongue: English
• Leaves: 314
• Series: Methods in Cell Biology 104
• Edition: 3
• Category: Library

✦ Synopsis

This volume on Genetics, Genomics, and Informatics, will cover new technologies in Forward and Reverse Genetics, Transgenesis, The Zebrafish Genome and Mapping Technologies, Informatics and Comparative Genomics, and Infrastructure. This volume of Methods in Cell Biology will prove valuable both to seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium.

✦ Table of Contents

Content:
Methods in Cell Biology
Pages i-iii

Copyright
Page iv

Contributors
Pages xiii-xvii

Preface
Pages xix-xx

Chapter 1 - Generating Conditional Mutations in Zebrafish Using Gene-trap Mutagenesis
Pages 1-22
Lisette A. Maddison, Jianjun Lu, Wenbiao Chen

Chapter 2 - Tol2-mediated Transgenesis, Gene Trapping, Enhancer Trapping, and the Gal4-UAS System
Pages 23-49
Gembu Abe, Maximilliano L. Suster, Koichi Kawakami

Chapter 3 - Engineering Zinc Finger Nucleases for Targeted Mutagenesis of Zebrafish
Pages 51-58
Jeffry D. Sander, J.-R. Joanna Yeh, Randall T. Peterson, J. Keith Joung

Chapter 4 - Retroviral-mediated Insertional Mutagenesis in Zebrafish
Pages 59-82
Adam Amsterdam, Gaurav Kumar Varshney, Shawn Michael Burgess

Chapter 5 - Genetic Screens for Mutations Affecting Adult Traits and Parental-effect Genes
Pages 83-120
Francisco Pelegri, Mary C. Mullins

Chapter 6 - High-Throughput Target-Selected Gene Inactivation in Zebrafish
Pages 121-127
Ross N.W. Kettleborough, Ewart de Bruijn, Freek van Eeden, Edwin Cuppen, Derek L. Stemple

Chapter 7 - Genetic Suppressor Screens in Haploids
Pages 129-136
Xiaoying Bai, Zhongan Yang, Hong Jiang, Shuo Lin, Leonard I. Zon

Chapter 8 - Transgenic Zebrafish Using Transposable Elements
Pages 137-149
Karl J. Clark, Mark D. Urban, Kimberly J. Skuster, Stephen C. Ekker

Chapter 9 - Spatiotemporal Control of Embryonic Gene Expression Using Caged Morpholinos
Pages 151-172
Ilya A. Shestopalov, James K. Chen

Chapter 10 - Advanced Zebrafish Transgenesis with Tol2 and Application for Cre/lox Recombination Experiments
Pages 173-194
Christian Mosimann, Leonard I. Zon

Chapter 11 - Use of Phage PhiC31 Integrase as a Tool for Zebrafish Genome Manipulation
Pages 195-208
James A. Lister

Chapter 12 - Method for Somatic Cell Nuclear Transfer in Zebrafish
Pages 209-217
Kannika Siripattarapravat, Jose B. Cibelli

Chapter 13 - Single Nucleotide Polymorphism (SNP) Panels for Rapid Positional Cloning in Zebrafish
Pages 219-235
Matthew D. Clark, Victor Guryev, Ewart de Bruijn, Isaac J. Nijman, Masazumi Tada, Catherine Wilson, Panos Deloukas, John H. Postlethwait, Edwin Cuppen, Derek L. Stemple

Chapter 14 - Molecular Cytogenetic Methodologies and a BAC Probe Panel Resource for Genomic Analyses in the Zebrafish
Pages 237-257
Kimberly P. Dobrinski, Kim H. Brown, Jennifer L. Freeman, Charles Lee

Chapter 15 - Conserved Synteny and the Zebrafish Genome
Pages 259-285
Julian M. Catchen, Ingo Braasch, John H. Postlethwait

Chapter 16 - The Zon Laboratory Guide to Positional Cloning in Zebrafish
Pages 287-309
Yi Zhou, Leonard I. Zon

Chapter 17 - Data Extraction, Transformation, and Dissemination through ZFIN
Pages 311-325
Douglas G. Howe, Ken Frazer, David Fashena, Leyla Ruzicka, Yvonne Bradford, Sridhar Ramachandran, Barbara J. Ruef, Ceri Van Slyke, Amy Singer, Monte Westerfield

Chapter 18 - DNA Methylation Profiling in Zebrafish
Pages 327-339
Shan-Fu Wu, Haiying Zhang, Saher Sue Hammoud, Magdalena Potok, David A. Nix, David A. Jones, Bradley R. Cairns

Chapter 19 - Chromatin Immunoprecipitation in Adult Zebrafish Red Cells
Pages 341-352
Eirini Trompouki, Teresa V. Bowman, Anthony DiBiase, Yi Zhou, Leonard I. Zon

Chapter 20 - Discerning Different In vivo Roles of MicroRNAs by Experimental Approaches in Zebrafish
Pages 353-378
Luke Pase, Graham J. Lieschke

Chapter 21 - Sequencing-based Expression Profiling in Zebrafish
Pages 379-399
Jin Liang, Gabriel Renaud, Shawn M. Burgess

Chapter 22 - Chromatin Modification in Zebrafish Development
Pages 401-428
Jordi Cayuso Mas, Emily S. Noël, Elke A. Ober

Chapter 23 - Advances in Zebrafish Husbandry and Management
Pages 429-451
Christian Lawrence

Chapter 24 - Aquaculture and Husbandry at the Zebrafish International Resource Center
Pages 453-478
Zoltán M. Varga

Index
Pages 479-493

Volumes in Series
Pages 495-504