The Zebrafish: Genetics and Genomics

✍ Scribed by H. William Detrich, Monte Westerfield and Leonard I. Zon (Eds.)

• Publisher: Academic Press
• Year: 1998
• Tongue: English
• Leaves: 428
• Series: Methods in Cell Biology 60
• Edition: 1st
• Category: Library

✦ Synopsis

This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features * Details state-of-the-art zebrafish protocols in a single-source reference * Presents methods and reagents in user-friendly format * Delineates critical steps and pitfalls of he procedures * Illustrates techniques with full-color plates * Summarizes many new and interesting developmental mutants * Includes appendices with strain information and a compendium of zebrafish World Wide Web sites * Relevant to clinicians interested in vertebrate models of human congenital diseases

✦ Table of Contents

Content:
Series Editors
Page ii

Edited by
Page iii

Copyright page
Page vi

Dedication
Page v

Contributors
Pages xiii-xv

Preface
Pages xvii-xviii
H. William Detrich III, Monte Westerfield, Leonard I. Zon

Chapter 1 A Gynogenesis-Based Screen for Maternal-Effect Genes in the Zebrafish Danio rerio Original Research Article
Pages 1-20
Francisco Pelegri, Stefan Schulte-Merker

Chapter 2 Developmental Mutant Screens in the Zebrafish Original Research Article
Pages 21-41
Fredericus J.M. van Eeden, Michael Granato, Jörg Odenthal, Pascal Haffter

Chapter 3 Haploid Screens and Gamma-Ray Mutagenesis Original Research Article
Pages 43-70
Charline Walker

Chapter 4 Early Pressure Screens Original Research Article
Pages 71-86
Christine E. Beattie, David W. Raible, Paul D. Henion, Judith S. Eisen

Chapter 5 Retrovirus-Mediated Insertional Mutagenesis in Zebrafish Original Research Article
Pages 87-98
Adam Amsterdam, Nancy Hopkins

Chapter 6 Genetic Applications of Transposons and Other Repetitive Elements in Zebrafish Original Research Article
Pages 99-131
Zoltán Ivics, Zsuzsanna Izsvák, Perry B. Hackett

Chapter 7 Transgenesis Original Research Article
Pages 133-148
Anming Meng, Jason R. Jessen, Shuo Lin

Chapter 8 The Zebrafish Genome Original Research Article
Pages 149-163
John Postlethwait, Angel Amores, Allan Force, Yi-Lin Yan

Chapter 9 Using Random Amplified Polymorphic DNAs in Zebrafish Genomic Analysis Original Research Article
Pages 165-179
John H. Postlethwait, Yi-Lin Yan, Michael A. Gates

Chapter 10 Simple Sequence-Length Polymorphism Analysis Original Research Article
Pages 181-183
Eric C. Liao, Leonard I. Zon

Chapter 11 Gene Mapping in Zebrafish Using Single-Strand Conformation Polymorphism Analysis Original Research Article
Pages 185-193
Dorothee Foernzler, David R. Beier

Chapter 12 Mapping Zebrafish Mutations by AFLP Original Research Article
Pages 195-211
David G. Ransom, Leonard I. Zon

Chapter 13 Zebrafish Expressed Sequence Tags and Their Applications Original Research Article
Pages 213-233
Zhiyuan Gong

Chapter 14 Zebrafish YAC, BAC, and PAC Genomic Libraries Original Research Article
Pages 235-258
Chris T. Amemiya, Tao P. Zhong, Gary A. Silverman, Mark C. Fishman, Leonard I. Zon

Chapter 15 Positional Cloning of Mutated Zebrafish Genes Original Research Article
Pages 259-286
William S. Talbot, Alexander F. Schier

Chapter 16 Construction and Characterization of Zebrafish Whole Genome Radiation Hybrids Original Research Article
Pages 287-302
Cheni Kwok, Ricky Critcher, Karin Schmitt

Chapter 17 Zebrafish/Mouse Somatic Cell Hybrids for the Characterization of the Zebrafish Genome Original Research Article
Pages 303-321
Marc Ekker, Fengchun Ye, Lucille Joly, Patricia Tellis, Mario Chevrette

Chapter 18 Banded Chromosomes and the Zebrafish Karyotype Original Research Article
Pages 323-338
Angel Amores, John H. Postlethwait

Chapter 19 Zebrafish Informatics and the ZFIN Database Original Research Article
Pages 339-355
Monte Westerfield, Eckehard Doerry, Arthur E. Kirkpatrick, Sarah A. Douglas

Appendix 1 Genetic Backgrounds and Some Standard Stocks and Strains Used in Zebrafish Developmental Biology and Genetics Original Research Article
Pages 357-359
Stephen L. Johnson, Leonard I. Zon

Appendix 2 Centromeric Markers in the Zebrafish Original Research Article
Pages 361-363
Donald A. Kane, Leonard I. Zon, H. William Detrich III

Appendix 3 Collection, Storage, and Use of Zebrafish Sperm Original Research Article
Pages 365-372
David G. Ransom, Leonard I. Zon

Appendix 4 Zebrafish Web Site Listings Original Research Article
Pages 373-385
Pat Edwards

Index
Pages 387-396

Colour Plate
Pages 397-407