The yeast 2 μ plasmid: strategies for the survival of a selfish DNA
✍ Scribed by Mead, David J. ;Gardner, David C. J. ;Oliver, Stephen G.
- Book ID
- 104729232
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 553 KB
- Volume
- 205
- Category
- Article
- ISSN
- 0026-8925
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Hybrid plasmids have been constructed containing segments of the yeast plasmid 2 μ DNA, the yeast ochre-suppressing SUP4.0 gene and the bacterial plasmid pBR322. Yeast transformation is detected with a host containing multiple ochre auxotrophic mutations. The transformed SUP4.0 gene is active and ca
Partially purified cell-free extracts prepared from growing cells of the yeast Saccharomyces cerevisiae catalyze DNA synthesis directed by the chimeric plasmid BTYP-2 containing the whole 2 μm DNA sequence. The biochemical properties of the reaction have been examined and inactivation studies indica