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Transpogenes: the transposition-like integration of short sequence DNA into the yeast 2μ, plasmid creates the STB locus and plasmid-size polymorphism

✍ Scribed by Rank, Gerald H.; Wei, Xiao; Pelcher, Lawrence E.

Book ID
122517028
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
779 KB
Volume
147
Category
Article
ISSN
0378-1119

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Yeast DNA pools were prepared by ligating partial Sau3A genomic digests from strains carrying various MAL genes into the BamHI site of the yeast-Escherichia coli shuttle vector YRp7. They were used to transform recipient yeast strains that could not utilize maltose since they lacked a classical MAL