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The xylA promoter of Bacillus megaterium mediates constitutive gene expression in Escherichia coli

✍ Scribed by Denise Knobloch; Andre Clemens; Kai Ostermann; Gerhard Rödel

Book ID
118283480
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
186 KB
Volume
11
Category
Article
ISSN
1618-0240

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We have cloned a 14 kb DNA segment containing the coding sequence (polC) for DNA polymerase III (PolIII) from the Bacillus subtilis chromosome. The plasmid carrying the sequence, pRO10, directs the synthesis of the 160 kDa PolIII protein and three additional polypeptides in Escherichia coli maxicell