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Expression of penicillin G acylase gene from Bacillus megaterium ATCC 14945 in Escherichia coli and Bacillus subtilis

✍ Scribed by Kang, Joo Hyun; Hwang, Young; Yoo, Ook Joon


Book ID
122702498
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
539 KB
Volume
17
Category
Article
ISSN
0168-1656

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Bifunctional recombinant plasmids were constructed, comprised of the E. coli vectors pBR322, pBR325 and pACYC184 and different plasmids from Gram-positive bacteria, e.g. pBSU161-1 of B. subtilis and pUB110 and pC221 of S. aureus. The beta-lactamase (bla) gene and the chloramphenicol acetyltransferas