Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945
✍ Scribed by Laura Martín; María A. Prieto; E. Cortés; JoséL. García
- Book ID
- 109320421
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 556 KB
- Volume
- 125
- Category
- Article
- ISSN
- 0378-1097
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A 4.3 kb EcoRI fragment carrying the gene for cytochrome P450meg, the steroid-15 beta-monooxygenase from Bacillus megaterium ATCC 13368, was cloned and completely sequenced. The gene codes for a protein of 410 amino acids and was expressed in Escherichia coli and B. subtilis. Protein extracts from t
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