The Virulence of Biochemical Mutants of Klebsiella pneumoniae
β Scribed by E. D. Garber, Adeline J. Hackett and R. Franklin
- Book ID
- 123669881
- Publisher
- National Academy of Sciences
- Year
- 1952
- Tongue
- English
- Weight
- 201 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/88490
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π SIMILAR VOLUMES
A series of mutants defective in nitrogen fixation (nif) were isolated in Klebsiella pneumoniae strain M5a1. The nif mutations were either located on plasmid pRD1 or on the K. pneumoniae chromosome. A total of 37 plasmid mutants and 28 chromosomal mutants were employed in complementation tests using
Polar mutations were obtained by integration of bacteriophage Mu c+ or Mu cts DNA into the Klebsiella pneumoniae nif genes located on plasmid pCE1, a derivative of pRD1. In addition, nif deletions were isolated from nif::Mu cts plasmids. Complementation data allowed the characterization of twelve ni