✦ LIBER ✦

The use of tritiated elastin for the determination of subnanogram amounts of elastase

✍ Scribed by P.J. Stone; G. Crombie; C. Franzblau

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 392 KB
Volume: 80
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90680-7

No coin nor oath required. For personal study only.

✦ Synopsis

A procedure to quantitate trace amounts of elastase in tissue or cell homogenate preparations is described. The procedure is a modification of a method employing NaB3H,-reduced elastin and it does not restrict assay volume. The assay is specific and can distinguish between pancreatic elastase and trypsin or chymotrypsin, both of which solubilize small amounts of the substrate. Pancreatic elastase remains active in this assay system for at least 4 weeks.

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