The use of trifluoroacetic acid in the reverse-phase liquid chromatography of peptides including secretin

✍ Scribed by D. Voskamp; C. Olieman; H. C. Beyerman

• Publisher: Elsevier Science
• Year: 2010
• Tongue: English
• Weight: 442 KB
• Volume: 99
• Category: Article
• ISSN: 0165-0513
• DOI: 10.1002/recl.19800990401

✦ Synopsis

Abstract

The reverse‐phase high‐performance liquid chromatography of underivatized peptides has been studied. The addition of trifluoroacetic acid — lipophilic ion‐pairing reagent — to the mobile phase gave excellent conditions for the analytical and preparative separation on octadecylsilyl‐silica of peptides varying in size from a 2‐peptide to a 32‐peptide (human calcitonin) inclusive.

The gastrointestinal 27‐peptide hormone secretin could thus be purified on a preparative scale and intramolecular rearrangements in hexapeptide fragments of secretin could be monitored in this way.