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Factors influencing the performance of peptide mapping by reversed-phase high-performance liquid chromatography

✍ Scribed by Michael W. Dong; An.D. Tran

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
947 KB
Volume
499
Category
Article
ISSN
1873-3778

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✦ Synopsis

Factors controlling

the performance of peptide mapping on reversed-phase columns were systematically evaluated. Performance criteria included resolution (peak capacity and selectivity), system reproducibility, sensitivity and analysis speed. Column configuration, characteristics of packing materials, mobile phase composition, operating variables and instrumental designs were found to influence the performance of peptide mapping. Considerations for peptide identification techniques are discussed.

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