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The use of thin acrylamide gels for DNA sequencing

โœ Scribed by F. Sanger; A.R. Coulson

Book ID
115908324
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
508 KB
Volume
87
Category
Article
ISSN
0014-5793

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๐Ÿ“œ SIMILAR VOLUMES

Use of solubilizable acrylamide disulfid
Use of solubilizable acrylamide disulfide gels for isolation of DNA fragments suitable for sequence analysis
โœ J.Norman Hansen ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 507 KB

Acrylamide gels which arc crosslinked by disultide bridges are found to be an excellent electrophoretic medium for DNA restriction fragments over a wide size range. The gels are solubilised by mercaptoethanol and the fragments are rapidly and conveniently recovered on DEAE~cellulose columns. Fragmen